1. Introduction to Protein Purification

  1. Jan-Christer Janson
  1. Bo Ersson1,
  2. Lars Rydén2 and
  3. Jan-Christer Janson3

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch1

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Ersson, B., Rydén, L. and Janson, J.-C. (2011) Introduction to Protein Purification, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch1

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. 1

    Medicago AB, Danmark-Berga 13, SE-755 98 Uppsala, Sweden

  2. 2

    Centre for Sustainable Development (CSD) Uppsala, Uppsala University, Villavägen 16, SE-752 36 Uppsala, Sweden

  3. 3

    Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • protein purification, methods and techniques - separation and purification of biological macromolecules, and advancements in bioscience and biotechnology;
  • fractionation techniques - complex protein mixtures fractionated by adsorption and precipitation methods;
  • fractionation monitoring - being proper, establishment of a reliable assay of biological activity

Summary

This chapter contains sections titled:

  • Introduction

  • The Protein Extract

  • An Overview of Fractionation Techniques

  • Fractionation Strategies

  • Monitoring the Fractionation

  • The Final Product

  • Laboratory Equipment

  • References