11. Affinity Ligands from Biological Combinatorial Libraries

  1. Jan-Christer Janson
  1. Per-Åke Nygren

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch11

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Nygren, P.-Å. (2011) Affinity Ligands from Biological Combinatorial Libraries, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch11

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. Division of Molecular Biotechnology, School of Biotechnology, Royal Institute of Technology (KTH), SE-106 91 Stockholm, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • affinity ligands from biological combinatorial libraries - advances, construction and handling of protein libraries, new routes for selective protein ligands;
  • choice of selection system, and choice of strategy - expression of library members, physical link between them and respective encoding nucleic acids;
  • recombinant factor VIII-binding cyclic peptide - selection, by phage display technology

Summary

This chapter contains sections titled:

  • Introduction

  • Design of Library

  • Selection Methods

  • Characterization and Further Engineering of Ligands

  • Applications

  • References