13. Refolding of Inclusion Body Proteins from E. coli

  1. Jan-Christer Janson
  1. Zhiguo Su1,
  2. Diannan Lu2 and
  3. Zheng Liu2

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch13

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Su, Z., Lu, D. and Liu, Z. (2011) Refolding of Inclusion Body Proteins from E. coli, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch13

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. 1

    State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China

  2. 2

    Department of Chemical Engineering, Tsinghua University, Beijing 100084, China

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • refolding of inclusion body proteins from E. coli - recombinant DNA technology, production of valuable foreign proteins at high expression rates and high concentrations;
  • refolding methods - in vitro refolding and refolded protein purification;
  • refolding by SEC, successful tool for protein purification - two key factors, loading of protein to column and change in protein size, renaturing during elution with refolding buffer

Summary

This chapter contains sections titled:

  • Introduction

  • Protein Folding In Vivo

  • Molecular Simulation of Protein Refolding and Aggregation

  • Refolding Methods

  • Applications

  • References