17. Two-Dimensional Electrophoresis in Proteomics

  1. Jan-Christer Janson
  1. Reiner Westermeier1 and
  2. Angelika Görg2

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch17

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Westermeier, R. and Görg, A. (2011) Two-Dimensional Electrophoresis in Proteomics, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch17

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. 1

    SERVA Electrophoresis GmbH, Carl-Benz-Strasse 7, D-69115 Heidelberg, Germany

  2. 2

    Department of Proteomics, Technische Universität München, D-85350 Freising-Weihenstephan, Germany

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • two-dimensional electrophoresis in proteomics;
  • SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE);
  • SDS electrophoresis - second dimension SDS PAGE, performed in the discontinuous tris-chloride/glycine buffer system, in presence of 0.1% SDS

Summary

This chapter contains sections titled:

  • Introduction

  • Brief Technology Review of 2D Electrophoresis

  • Sample Preparation

  • Isoelectric Focusing

  • SDS Electrophoresis

  • Protein Detection

  • Image Analysis

  • Strategy for Protein Mapping in Proteomics

  • Protein Identification and Characterization

  • References