18. Protein Elution and Blotting Techniques

  1. Jan-Christer Janson
  1. Reiner Westermeier

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch18

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Westermeier, R. (2011) Protein Elution and Blotting Techniques, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch18

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. SERVA Electrophoresis GmbH, Carl-Benz-Strasse 7, D-69115 Heidelberg, Germany

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • protein elution and blotting techniques;
  • protein blotting, transfer of large molecules - onto surface of immobilizing membrane;
  • ion-exchange membranes, diethylaminoethyl (DEAE) - or carboxymethyl (CM) for preparative purposes, and ionic bond reversibility

Summary

This chapter contains sections titled:

  • Introduction

  • Protein Elution

  • Protein Blotting

  • References