20. High Throughput Screening Techniques in Protein Purification

  1. Jan-Christer Janson
  1. Karol M. Lacki and
  2. Eggert Brekkan

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch20

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Lacki, K. M. and Brekkan, E. (2011) High Throughput Screening Techniques in Protein Purification, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch20

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. GE Healthcare Bio-Sciences AB, SE-751 84 Uppsala, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • separation method optimization - high throughput screening techniques in protein purification, high throughput methods, in development of industrial scale protein purification processes;
  • qualitative comparison of HT methods - development of chromatographic separations;
  • Dedicated Design of Experiments (DOE) software - Design Expert and MODDE, planning of experiments and evaluation of results

Summary

This chapter contains sections titled:

  • Introduction

  • Technical Overview

  • Microtiter-Based High Throughput Method: Experimental Principle

  • Implementation of Design of Experiments in the High Throughput Process Development (HTPD) Area

  • Analytics

  • Applications

  • Summary and Future Trends

  • References