5. High-Resolution Reversed-Phase Chromatography of Proteins

  1. Jan-Christer Janson
  1. Sylvia Winkel Pettersson

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch5

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Pettersson, S. W. (2011) High-Resolution Reversed-Phase Chromatography of Proteins, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch5

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. Chromatography and Applications, Eka Chemicals AB/Akzo Nobel, Bohus, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • High-Resolution Reversed-Phase Chromatography of Proteins, and Reversed phase high-performance liquid chromatography (RP-HPLC, as RPC) - often-used technique in protein research;
  • flow chart for choosing right method of peak identification - and flow chart for development/optimization of analytical separations of smaller peptides;
  • preparative RPC, process used for purification - costly pharmaceutical products, isolation of microgram quantities of enzymes, insulin, in preparative applications

Summary

This chapter contains sections titled:

  • Introduction

  • Fundamentals of RPC

  • Development and Optimization of Analytical Separations

  • Development and Optimization of Preparative Separations

  • Scale-Up

  • Column Packing Material

  • Column Design

  • Practical Recommendations: How to Avoid and Solve Problems

  • References