6. Hydrophobic Interaction Chromatography

  1. Jan-Christer Janson
  1. Kjell-Ove Eriksson and
  2. Makonnen Belew

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch6

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Eriksson, K.-O. and Belew, M. (2011) Hydrophobic Interaction Chromatography, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch6

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. GE Healthcare Bio-Sciences AB, R&D Department, SE-751 84 Uppsala, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • hydrophobic interaction chromatography (HIC) - interaction of molecules with each other which is stronger, unaccounted for by covalent, or charge-transfer forces;
  • charged type of HIC adsorbents – controversies, mechanisms underlying binding of proteins to these media;
  • purification of alcohol dehydrogenase (ADH) by Expanded Bed Adsorption (EBA) - using a streamline-phenyl media, direct recovery of ADH from yeast cell homogenate

Summary

This chapter contains sections titled:

  • Introduction

  • History

  • Nomenclature

  • RPC vs HIC

  • Theory

  • Factors that Impact HIC

  • Experimental Design

  • Applications

  • References