9. Affinity Chromatography

  1. Jan-Christer Janson
  1. Francisco Batista-Viera1,
  2. Jan-Christer Janson3 and
  3. Jan Carlsson2

Published Online: 11 MAR 2011

DOI: 10.1002/9780470939932.ch9

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

How to Cite

Batista-Viera, F., Janson, J.-C. and Carlsson, J. (2011) Affinity Chromatography, in Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition (ed J.-C. Janson), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470939932.ch9

Editor Information

  1. Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Author Information

  1. 1

    Cátedra de Bioquímica, Dpto. de Biociencias, Facultad de Quimica Gral. Flores 2124. Casilla de Correo 1157, Montevideo, Uruguay

  2. 2

    Department of Physical & Analytical Chemistry, Uppsala University, Box 579, SE-751 23 Uppsala, Sweden

  3. 3

    Department of Physical and Analytical Chemistry, Uppsala University, Box 579, S-751 23 Uppsala, Sweden

Publication History

  1. Published Online: 11 MAR 2011
  2. Published Print: 7 MAR 2011

ISBN Information

Print ISBN: 9780471746614

Online ISBN: 9780470939932

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Keywords:

  • affinity chromatography, all kinds of adsorption chromatographies - and nontraditional ligands in the extreme, all chromatographies except ion exchange;
  • affinity interactions - a good affinity ligand - with certain characteristics;
  • typical separation by affinity chromatography - four stages: adsorption, washing, elution, and column regeneration

Summary

This chapter contains sections titled:

  • Introduction

  • Affinity Interactions

  • Preparation and Evaluation of Affinity Adsorbents

  • Immobilization Techniques

  • Chromatographic Techniques

  • Applications

  • References