3. Sample Preparation Variables in Platelet Proteomics for Biomarker Research

  1. Ángel García3 and
  2. Yotis A. Senis4
  1. Maria Zellner1 and
  2. Rudolf Oehler2

Published Online: 12 JUL 2011

DOI: 10.1002/9780470940297.ch3

Platelet Proteomics: Principles, Analysis, and Applications

Platelet Proteomics: Principles, Analysis, and Applications

How to Cite

Zellner, M. and Oehler, R. (2011) Sample Preparation Variables in Platelet Proteomics for Biomarker Research, in Platelet Proteomics: Principles, Analysis, and Applications (eds Á. García and Y. A. Senis), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470940297.ch3

Editor Information

  1. 3

    Department of Pharmacology, Facultade de Farmacia, Universidade de Santiago de Compostela, Santiago de Compostela, Spain

  2. 4

    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

Author Information

  1. 1

    Institute of Physiology, Center for Physiology and Pharmacology, Medical University Vienna, Vienna, Austria

  2. 2

    Department of Surgery, Center of Translational Research, Medical University of Vienna, Vienna, Austria

Publication History

  1. Published Online: 12 JUL 2011
  2. Published Print: 9 MAY 2011

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio5 and
  2. Nico M. M. Nibbering6

Series Editor Information

  1. 5

    Departments of Neurology and Biochemistry, University of Tennessee Health Science Center, USA

  2. 6

    Vrije Universiteit Amsterdam, The Netherlands

ISBN Information

Print ISBN: 9780470463376

Online ISBN: 9780470940297

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Keywords:

  • sample preparation variables, platelet proteomics - biomarker research, preparing platelets;
  • cell lysis, protein sample preparation - plasma-free platelets, affecting platelet proteomic analysis;
  • variations, platelet separation from plasma - platelet-associated plasma proteins

Summary

This chapter contains sections titled:

  • Introduction

  • Selection of Test Subjects and Blood Collection

  • Blood Sampling

  • Platelet Purification

  • Separation from Plasma Proteins

  • Cell Lysis

  • Concluding Remarks

  • Acknowledgments

  • References