5. The Platelet Membrane Proteome

  1. Ángel García1 and
  2. Yotis A. Senis2
  1. Yotis A. Senis

Published Online: 12 JUL 2011

DOI: 10.1002/9780470940297.ch5

Platelet Proteomics: Principles, Analysis, and Applications

Platelet Proteomics: Principles, Analysis, and Applications

How to Cite

Senis, Y. A. (2011) The Platelet Membrane Proteome, in Platelet Proteomics: Principles, Analysis, and Applications (eds Á. García and Y. A. Senis), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470940297.ch5

Editor Information

  1. 1

    Department of Pharmacology, Facultade de Farmacia, Universidade de Santiago de Compostela, Santiago de Compostela, Spain

  2. 2

    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

Author Information

  1. Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

Publication History

  1. Published Online: 12 JUL 2011
  2. Published Print: 9 MAY 2011

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio3 and
  2. Nico M. M. Nibbering4

Series Editor Information

  1. 3

    Departments of Neurology and Biochemistry, University of Tennessee Health Science Center, USA

  2. 4

    Vrije Universiteit Amsterdam, The Netherlands

ISBN Information

Print ISBN: 9780470463376

Online ISBN: 9780470940297

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Keywords:

  • platelet membrane proteome - all proteins, associated with platelet lipid bilayers;
  • protein separation strategies, for protein separation prior to MS - reducing sample complexity, identifying low-abundance IMP and signaling proteins;
  • peripheral membrane protein identification - platelet PM, changes following platelet activation

Summary

This chapter contains sections titled:

  • Introduction: Classification of Membrane Proteins

  • Challenges of Membrane Proteomics

  • Enriching Membrane Proteins

  • Protein Separation Strategies

  • Shotgun Strategies for Identifying Integral Membrane Proteins

  • Mapping the Platelet Membrane Proteome

  • Identifying Peripheral Membrane Proteins in Activated Platelets

  • Future Directions

  • Concluding Remarks

  • Acknowledgments

  • References