9. Serial Analysis of Gene Expression (SAGE) for Studying the Platelet and Megakaryocyte Transcriptome

  1. Ángel García2 and
  2. Yotis A. Senis3
  1. Michael G. Tomlinson

Published Online: 12 JUL 2011

DOI: 10.1002/9780470940297.ch9

Platelet Proteomics: Principles, Analysis, and Applications

Platelet Proteomics: Principles, Analysis, and Applications

How to Cite

Tomlinson, M. G. (2011) Serial Analysis of Gene Expression (SAGE) for Studying the Platelet and Megakaryocyte Transcriptome, in Platelet Proteomics: Principles, Analysis, and Applications (eds Á. García and Y. A. Senis), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9780470940297.ch9

Editor Information

  1. 2

    Department of Pharmacology, Facultade de Farmacia, Universidade de Santiago de Compostela, Santiago de Compostela, Spain

  2. 3

    Centre for Cardiovascular Sciences, Institute of Biomedical Research, School of Clinical and Experimental Medicine, College of Medical and Dental Sciences, University of Birmingham, Birmingham, United Kingdom

Author Information

  1. School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, United Kingdom

Publication History

  1. Published Online: 12 JUL 2011
  2. Published Print: 9 MAY 2011

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio4 and
  2. Nico M. M. Nibbering5

Series Editor Information

  1. 4

    Departments of Neurology and Biochemistry, University of Tennessee Health Science Center, USA

  2. 5

    Vrije Universiteit Amsterdam, The Netherlands

ISBN Information

Print ISBN: 9780470463376

Online ISBN: 9780470940297

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Keywords:

  • integrated “omics,” in disease - Serial Analysis of Gene Expression (SAGE), platelet and megakaryocyte transcriptome study;
  • SAGE method, overcoming limitations of EST sequencing - DNA microarrays for transcriptome characterization;
  • combining SAGE with next-generation sequencing - 3′-tag digital gene expression (DGE), sequencing individual LongSAGE tags

Summary

This chapter contains sections titled:

  • Introduction

  • A Sage Discovery

  • Longsage and Supersage Improvements

  • Platelet Sage

  • Megakaryocyte Sage

  • Next-Generation Sequencing

  • RNA-Seq

  • Combining Sage with Next-Generation Sequencing

  • Concluding Remarks

  • Acknowledgments

  • References