Unit

Genetic Modifier Screens in Mice

  1. Marina R. Carpinelli1,3,4,5,
  2. Rachel A. Burt1,3,4,5,
  3. Benjamin T. Kile2,3

Published Online: 1 MAR 2012

DOI: 10.1002/9780470942390.mo110161

Current Protocols in Mouse Biology

Current Protocols in Mouse Biology

How to Cite

Carpinelli, M. R., Burt, R. A. and Kile, B. T. 2012. Genetic Modifier Screens in Mice. Current Protocols in Mouse Biology. 2:75–87.

Author Information

  1. 1

    Molecular Medicine Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

  2. 2

    Cancer and Hematology Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia

  3. 3

    Department of Medical Biology, The University of Melbourne, Parkville, Australia

  4. 4

    Hearing Co-Operative Research Centre, Melbourne, Australia

  5. 5

    Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Australia

Publication History

  1. Published Online: 1 MAR 2012
  2. Published Print: MAR 2012

Abstract

ENU mutagenesis is a forward genetics strategy in which random mutagenesis and phenotypic screening is used to identify genes based on the phenotype induced when they are mutated. A modifier screen is a type of screen in which mice with a pre-existing phenotype are utilized to identify mutations that can enhance or suppress this phenotype. This approach has the potential to uncover missing pathway members, reveal novel genetic interactions, and pinpoint new drug targets. Considerations when planning a suppressor screen include current knowledge, genomic footprint, penetrance, variance, robustness, latency of the starting phenotype, viability, fertility, genetic background and ENU tolerance of starting strain, screening assay, mouse numbers required, and mutation identification strategy. Practical advice on each of these is provided in this review. Curr. Protoc. Mouse Biol. 2:75-87 © 2012 by John Wiley & Sons, Inc.

Keywords:

  • ENU mutagenesis;
  • modifier screen;
  • functional genetics