3. Next Generation DNA Sequencing Technologies and Applications

  1. Zhanjiang (John) Liu
  1. Qingshu Meng and
  2. Jun Yu

Published Online: 10 DEC 2010

DOI: 10.1002/9780470958964.ch3

Next Generation Sequencing and Whole Genome Selection in Aquaculture

Next Generation Sequencing and Whole Genome Selection in Aquaculture

How to Cite

Meng, Q. and Yu, J. (2011) Next Generation DNA Sequencing Technologies and Applications, in Next Generation Sequencing and Whole Genome Selection in Aquaculture (ed Z. (. Liu), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470958964.ch3

Editor Information

  1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849 USA

Author Information

  1. CAS Key Laboratory of Genome Science and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, China

Publication History

  1. Published Online: 10 DEC 2010
  2. Published Print: 28 JAN 2011

ISBN Information

Print ISBN: 9780813806372

Online ISBN: 9780470958964

SEARCH

Keywords:

  • Next Generation DNA sequencing technologies and applications;
  • DNA sequencing, undoubtedly the most important technology in biology;
  • beneficiaries of rapidly advancing DNA technology - understanding critical points, taking advantage of situation;
  • wrong decisions, costly since unit prices of sequencing machines - in range of half a million U.S. dollars;
  • next generation DNA sequencing platforms - relative strengths and limitations, and their applications;
  • Next Generation sequencing - The Current Platforms, The Roche (454) Genome Sequencer FLX System;
  • major limitation of 454 technology - and resolution of homopolymer-containing DNA segments;
  • Illumina sequencing-by-synthesis approach;
  • genome de novo sequencing and assembly;
  • RNA-Seq, recently developed approach - for transcriptome profiling, using deep sequencing technologies

Summary

This chapter contains sections titled:

  • Introduction

  • Next Generation Sequencing: The Current Platforms

  • Application of Next Generation Sequencing Technologies

  • Next - Next Generation Sequencing

  • Perspectives

  • References