4. Library Construction for next Generation Sequencing

  1. Zhanjiang (John) Liu
  1. Huseyin Kucuktas and
  2. Zhanjiang (John) Liu

Published Online: 10 DEC 2010

DOI: 10.1002/9780470958964.ch4

Next Generation Sequencing and Whole Genome Selection in Aquaculture

Next Generation Sequencing and Whole Genome Selection in Aquaculture

How to Cite

Kucuktas, H. and Liu, Z. (2011) Library Construction for next Generation Sequencing, in Next Generation Sequencing and Whole Genome Selection in Aquaculture (ed Z. (. Liu), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470958964.ch4

Editor Information

  1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849 USA

Author Information

  1. The Fish Molecular Genetics and Biotechnology Laboratory, Department of Fisheries and Allied Aquacultures and Program of Cell and Molecular Biosciences, Aquatic Genomics Unit, Auburn University, Auburn, AL 36849 USA

Publication History

  1. Published Online: 10 DEC 2010
  2. Published Print: 28 JAN 2011

ISBN Information

Print ISBN: 9780813806372

Online ISBN: 9780470958964

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Keywords:

  • library construction for next generation sequencing;
  • Illumina sequencing platform, sample preparation - and sources of DNA or RNA and purpose of sequencing project;
  • sample preparation for Illumina sequencing;
  • general procedures of library preparation - for next generation sequencing;
  • DNA fragmentation, first step of DNA sample preparation - generating fragments of various sizes;
  • sonication, shears DNA into small fragments - use of hydrodynamic force using a sonicator;
  • DNA fragmentation, through use of enzymatic fragmentation - as New England Biolabs (NEB) fragmentase;
  • DNA end repair, problems of DNA after fragmentation - need for end polishing;
  • indexed libraries, sequencing multiple samples in a single lane;
  • sample preparation for sequencing multiplexed samples - using SOLiD sequencing

Summary

This chapter contains sections titled:

  • Sample Preparation for Illumina Sequencing

  • Indexed Libraries for Sequencing Multiple Samples in a Single Lane

  • Sample Preparation for 454 Sequencing

  • Sample Preparation for Sequencing Multiplexed Samples Using SOLiD Sequencing

  • Acknowledgments

  • References