3. Protein Separation Strategies

  1. P. David Eckersall2 and
  2. Phillip D. Whitfield3
  1. Ingrid Miller

Published Online: 5 AUG 2011

DOI: 10.1002/9780470960660.ch3

Methods in Animal Proteomics

Methods in Animal Proteomics

How to Cite

Miller, I. (2011) Protein Separation Strategies, in Methods in Animal Proteomics (eds P. D. Eckersall and P. D. Whitfield), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470960660.ch3

Editor Information

  1. 2

    University of Glasgow, UK

  2. 3

    University of the Highlands and Islands, UK

Author Information

  1. University of Veterinary Medicine, Vienna, Austria

Publication History

  1. Published Online: 5 AUG 2011
  2. Published Print: 19 AUG 2011

ISBN Information

Print ISBN: 9780813817910

Online ISBN: 9780470960660



  • protein separation strategies - proteomics, and simultaneous analysis of complex protein mixtures;
  • proteomic investigations, and protein patterns - protein identification, achieved at a later time;
  • 2DE and associated protein separation strategies - methodological possibilities offered;
  • “Classical” 2DE - typical 2DE experiment - of unknown, or little characterized sample starting with a standard protocol;
  • other varieties of 2DE - different additives and separation conditions;
  • precautions, taken when separating - alkaline proteins in wide-range IPGs;
  • denaturing versus native 2DE - native structure of protein, determined by its sequence;
  • Blue Native (BN-) PAGE and Clear Native (CN-) PAGE - denaturing protein sample;
  • protein precipitation, an easy way - of removing nonprotein contaminants from sample;
  • 2DE, high-resolution and versatile method - analysis of proteomic questions


This chapter contains sections titled:

  • Introduction

  • “Classical” 2 DE

  • Other Varieties of 2 DE

  • Sample Complexity

  • The Influence of Staining

  • “Post - detection”

  • Future Trends

  • Further Reading

  • References