6. Comparative Proteomic Approaches

  1. P. David Eckersall2 and
  2. Phillip D. Whitfield3
  1. Johanna S. Rees and
  2. Kathryn S. Lilley

Published Online: 5 AUG 2011

DOI: 10.1002/9780470960660.ch6

Methods in Animal Proteomics

Methods in Animal Proteomics

How to Cite

Rees, J. S. and Lilley, K. S. (2011) Comparative Proteomic Approaches, in Methods in Animal Proteomics (eds P. D. Eckersall and P. D. Whitfield), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470960660.ch6

Editor Information

  1. 2

    University of Glasgow, UK

  2. 3

    University of the Highlands and Islands, UK

Author Information

  1. University of Cambridge, UK

Publication History

  1. Published Online: 5 AUG 2011
  2. Published Print: 19 AUG 2011

ISBN Information

Print ISBN: 9780813817910

Online ISBN: 9780470960660



  • comparative proteomic approaches - organisms at systems-wide level, a holistic view;
  • proteins, work horses of cell - knowledge of expression levels, interaction partners and subcellular location;
  • simplest proteomics experiment - catalog of proteins, present in any sample;
  • complex proteomes, analyzed with simple cell lysate - giving rise to isoforms of proteins;
  • phosphoprotein and phosphopeptide detection - a fast-growing field, in MS;
  • collision induced dissociation (CID) within tandem MS - and loss of phosphate moiety from phosphothreonine and phosphoserine;
  • protein methylation - critical process in gene transcription, activation and repression;
  • ubiquitination (Ub), factor in protein turnover - implication in diseases;
  • organelle protein localization, by isotope tagging (LOPIT) - gradient-based approach, inspired by de Duve's observations;
  • bioinformatics and biostatistics - proteomics technologies, reliant on bioinformatics methodologies, protein species identification


This chapter contains sections titled:

  • Global Study of Proteins

  • Approaches to Characterize the Proteome

  • Quantitative Proteomics

  • Bioinformatics and Biostatistics

  • Future Prospects

  • References