8. Proteomic Strategies to Investigate Adaptive Processes

  1. P. David Eckersall2 and
  2. Phillip D. Whitfield3
  1. L. Elaine Epperson and
  2. Sandra L. Martin

Published Online: 5 AUG 2011

DOI: 10.1002/9780470960660.ch8

Methods in Animal Proteomics

Methods in Animal Proteomics

How to Cite

Epperson, L. E. and Martin, S. L. (2011) Proteomic Strategies to Investigate Adaptive Processes, in Methods in Animal Proteomics (eds P. D. Eckersall and P. D. Whitfield), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780470960660.ch8

Editor Information

  1. 2

    University of Glasgow, UK

  2. 3

    University of the Highlands and Islands, UK

Author Information

  1. University of Colorado School of Medicine, CO, USA

Publication History

  1. Published Online: 5 AUG 2011
  2. Published Print: 19 AUG 2011

ISBN Information

Print ISBN: 9780813817910

Online ISBN: 9780470960660

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Keywords:

  • proteomics in animal biology - investigating adaptive processes;
  • living organisms, subject to changes - in environment, challenges met by altering protein expression and enzymatic activities;
  • unique challenges, arising - questions pertaining to behavioral adaptation of animals;
  • hibernation physiology and behavior - mammalian hibernation and circannual rhythm;
  • two-switch model of hibernation - shifts in biomolecules, physiological and behavioral cycles of hibernation;
  • robust sampling strategy - assessing changes in adaptive processes, critical phenotypic features in natural behavioral cycle;
  • hibernation, like other adaptive phenotypes - environmental cues for maintenance and transitions;
  • 2D gels in combination, with fluorescent Cy dyes (DIGE; GE Healthcare) - internal reference for normalization common to every gel;
  • proper statistical analysis - critical for arriving at accurate and meaningful results;
  • label and gel-free proteomic approach - investigating hibernation in liver of arctic ground squirrels

Summary

This chapter contains sections titled:

  • Introduction

  • Hibernation Physiology and Behavior

  • Sampling Strategy

  • Sample Collection and Storage

  • Design and Execution of a Quantitative Proteomic Experiment

  • Conclusion

  • References