19. Xylooligosaccharides Production, Quantification, and Characterization in Context of Lignocellulosic Biomass Pretreatment

  1. Charles E. Wyman4,5
  1. Qing Qing1,
  2. Hongjia Li4,5,†,
  3. Rajeev Kumar2,5 and
  4. Charles E. Wyman4,5

Published Online: 5 APR 2013

DOI: 10.1002/9780470975831.ch19

Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals

Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals

How to Cite

Qing, Q., Li, H., Kumar, R. and Wyman, C. E. (2013) Xylooligosaccharides Production, Quantification, and Characterization in Context of Lignocellulosic Biomass Pretreatment, in Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals (ed C. E. Wyman), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470975831.ch19

Editor Information

  1. 4

    Department of Chemical and Environmental Engineering and Center for Environmental Research and Technology, University of California, Riverside, USA

  2. 5

    BioEnergy Science Center, Oak Ridge, USA

Author Information

  1. 1

    Pharmaceutical Engineering & Life Science, Changzhou University, Changzhou, China

  2. 2

    Center for Environmental Research and Technology, University of California, Riverside, USA

  3. 4

    Department of Chemical and Environmental Engineering and Center for Environmental Research and Technology, University of California, Riverside, USA

  4. 5

    BioEnergy Science Center, Oak Ridge, USA

  1. DuPont Industrial Biosciences, Palo Alto, USA

Publication History

  1. Published Online: 5 APR 2013
  2. Published Print: 10 MAY 2013

Book Series:

  1. Wiley Series in Renewable Resources

Book Series Editors:

  1. Christian V. Stevens

Series Editor Information

  1. Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium

ISBN Information

Print ISBN: 9780470972021

Online ISBN: 9780470975831

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Keywords:

  • characterization;
  • degree of polymerization;
  • hydrolysis;
  • pretreatment;
  • xylooligosaccharides

Summary

Xylooligosaccharides (XOs) released during hydrothermal pretreatment, low-severity dilute-acid pretreatment, or enzymatic hydrolysis of lignocellulosic biomass can be fermented directly, further hydrolyzed into fermentable sugars for biofuels production, or used immediately as high-value-added prebiotic feedstocks which can be incorporated into many food products. Quantification and detailed characterization of xylooligosaccharides are vital to understand reaction kinetics and sugar-release mechanisms during pretreatment and enzymatic hydrolysis, clarify cell-wall structural recalcitrance, and facilitate XOs as important prebiotic products. This chapter reviews chemical and enzymatic methods for production of xylooligosaccharide mixtures as well as separation and purification processes. In addition, characterization methods such as by high-pressure liquid chromatography (HPLC), high-performance anion exchange chromatography (HPAEC), mass spectrometry (MS), and nuclear magnetic resonance (NMR) are summarized for quantitative determination and structural characterization of xylooligosaccharides.