11. Biophysics/Label-Free Assays in Hit Discovery and Verification

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Johannes Ottl

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch11

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Ottl, J. (2011) Biophysics/Label-Free Assays in Hit Discovery and Verification, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch11

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. Novartis Institute for BioMedical Research, Centre for Proteomic Chemistry, Forum 1, Novartis Campus, CH-4056, Basel, Switzerland

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • biophysics/label-free assays - in hit discovery and verification;
  • drug discovery, dramatic changes - in early drug research and discovery process;
  • high throughput screening (HTS) - main methods for generating leads for drug discovery;
  • application of biophysics in academic environment - well-established;
  • quests of lead discovery program teams for biophysics;
  • HTS verification - the key task of biophysics;
  • biophysical methods, tool for validating molecular interactions - determining kinetic and structural parameters for molecular recognition by protein targets;
  • biophysics toolbox, and right biophysics tools - placed at a suitable place in drug discovery assay project flowchart;
  • biophysics technologies - applied in hit and lead finding;
  • affinity MS screening assay set-up

Summary

This chapter contains sections titled:

  • Introduction

  • Why Biophysics?

  • Biophysics/Label-Free Toolbox

  • Which Biophysical Measurement at Which Stage of a Drug Discovery Project Flowchart?

  • Examples of Higher Throughput Biophysics Applied in Hit and Lead Finding

  • Conclusion

  • Outlook

  • References