13. Use of Label-Free Detection Technologies in the Hit-to-Lead Process: Surface Optical Detection of Cellular Processes

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. F. Stuhmeier,
  2. J. Bradley,
  3. E. Fairman,
  4. E. Gbekor,
  5. P. Hayter and
  6. S. Ramsey

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch13

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Stuhmeier, F., Bradley, J., Fairman, E., Gbekor, E., Hayter, P. and Ramsey, S. (2011) Use of Label-Free Detection Technologies in the Hit-to-Lead Process: Surface Optical Detection of Cellular Processes, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch13

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. Pfizer Global Research and Development, Sandwich, Kent CT13 9NJ, UK

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • use of label-free detection technologies - in hit-to-lead process, surface optical detection of cellular processes;
  • hit-to-lead optimization process - dominated by use of highly purified enzymes and proteins;
  • label-free assays for plate-based screening - advantages over established assay methods;
  • label-free methods, revolutionizing - field of ion channel primary screening and SAR generation;
  • 384 version of CellKey™ system - impedance system, for higher throughput SAR generation in 384 format;
  • Corning Epic™ reader - technically similar to SRU Biosystems Bind™ reader;
  • surface optical detection - of cellular processes;
  • assay quality, influenced by every factor - change in index of refraction of assay solvent;
  • label-free systems for cellular assays - not part of mainstream drug discovery process

Summary

This chapter contains sections titled:

  • Introduction

  • Overview of Label-Free Assay Platforms

  • Surface Optical Detection of Cellular Processes

  • Discussion

  • References