14. Cellular Screening for 7TM Receptors Using Label-Free Detection

  1. Matthew Cooper4 and
  2. Lorenz M. Mayr5
  1. Jeffrey C. Jerman1,
  2. Jason Brown2 and
  3. Magalie Rocheville3

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch14

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Jerman, J. C., Brown, J. and Rocheville, M. (2011) Cellular Screening for 7TM Receptors Using Label-Free Detection, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch14

Editor Information

  1. 4

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 5

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. 1

    Molecular Discovery Research, 1–3 Burtonhole Lane, London NW7 1AD, UK

  2. 2

    Neurosciences Centre of Excellence in Drug Discovery, GlaxoSmith-Kline, New Frontiers Science Park, Harlow, CM19 5AW, UK

  3. 3

    Molecular Discovery Research, GlaxoSmithKline, New Frontiers Science Park, Harlow, CM19 5AW, UK

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • cellular screening for 7TM receptors - using label-free detection;
  • label-free detection, a powerful approach - study of molecular interactions without requirement for target modification;
  • readout, noninvasive, cumulative and signaling-route independent;
  • kinetic responses or ‘fingerprints’ - elicited by a compound, mechanistically informative;
  • label-free assays on BIND® and CellKey™ platforms - using S1P1 recombinant cell line;
  • data, compared to that from alternative assay formats - and fluorescence imaging plate reader (FLIPR);
  • choice of assay format - for compound identification and optimisation;
  • Hit identification - U2OS cells, endogenously expressing histamine H1 receptor;
  • CellKey™ Label-Free Assay Protocol;
  • BIND® Label-Free Assay Protocol

Summary

This chapter contains sections titled:

  • Introduction

  • Results and Discussion

  • Conclusions and Perspective

  • Materials and Methods

  • Acknowledgements

  • References