18. SPR-Based Direct Binding Assays in Drug Discovery

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Walter Huber

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch18

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Huber, W. (2011) SPR-Based Direct Binding Assays in Drug Discovery, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch18

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. F.Hoffmann-La RocheAG, Pharma Research Basel, Grenzacherstrasse, 4070 Basel, Switzerland

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129



  • SPR-based direct binding assays - in drug discovery;
  • screening using SPR-based direct binding assay - pharmaceutical industry, exploring new and innovative methods, discovering small molecule drug candidates;
  • high quality hit and lead compound identification - in discovery of new drug molecules;
  • single concentration affinity filter - measured responses at given concentration;
  • selection filters, and respective assay types - in screening assays;
  • ratio of responses, at two different concentrations - early filter criterion for selection of positive hits;
  • assay set-up - SPR measurements, performed on a Biacore S51 instrument;
  • lead selection, using SPR-based binding assay - interaction of potential lead candidates


This chapter contains sections titled:

  • Introduction

  • Screening Using SPR-Based Direct Binding Assay

  • Lead Selection Using SPR-Based Binding Assay

  • Conclusion

  • Acknowledgements

  • References