20. ITC: More Than Just Binding Affinities

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Ernesto Freire

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch20

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Freire, E. (2011) ITC: More Than Just Binding Affinities, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch20

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129



  • ITC - more than just binding affinities;
  • isothermal titration calorimetry (ITC) - gold standard, for measuring binding affinities;
  • standard ITC titration experiments - binding reaction, injection of second reactant into reaction cell;
  • heat associated with each injection - proportional to amount of complex formed;
  • experimental observable in ITC - heat of reaction, binding enthalpy and entropy for reaction;
  • enthalpy and entropy, forces of binding - two forces, determining ligand to target binding;
  • thermodynamic signatures - for rosuvastatin binding to HMG-CoA reductase;
  • thermodynamic signature - of candidate for optimization, measured by ITC;
  • ITC, measuring binding enthalpy - and binding entropy, tool for optimization of drug candidates


This chapter contains sections titled:

  • Introduction

  • Why Should We Care About Enthalpy and Entropy?

  • Conclusion

  • Acknowledgements

  • References