3. SPR Screening of Chemical Microarrays for Fragment-Based Discovery

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Thomas Neumann and
  2. Renate Sekul

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch3

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Neumann, T. and Sekul, R. (2011) SPR Screening of Chemical Microarrays for Fragment-Based Discovery, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch3

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. Graffinity Pharmaceuticals GmbH, INF 518, 69120 Heidelberg, Germany

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129



  • SPR screening of chemical microarrays - for fragment-based discovery;
  • surface plasmon resonance (SPR), versatile tool - and study of biomolecular interactions;
  • fragment screening, and fragments - drug-like substances, interacting with protein epitopes or subsites;
  • SPR fragment screening - highly sensitive screening technologies, detection of weak protein fragment interactions;
  • library compounds, synthesized - using parallel high throughput chemistry approaches;
  • parallel synthesis of library compounds;
  • library design and array content - probability of hit identification;
  • chemical microarray production;
  • SPR imaging, and chemical microarrays - read using Graffinity's proprietary Plasmon Imager® for label-free imaging;
  • SPR measurement data of affinity fingerprint experiments - in two dimensional false colour-coded interaction map


This chapter contains sections titled:

  • Introduction

  • Key Features of Fragment Screening

  • SPR Fragment Screening

  • Synthesis of Library Compounds

  • Library Design and Array Content

  • Chemical Microarray Production

  • Surface Plasmon Resonance

  • SPR Imaging

  • Array Visualization and Analysis

  • Follow-up

  • Applications: MMP Case Study

  • Other Target Classes

  • Conclusion

  • References