5. Dynamic and Label-Free Cell-Based Assays Using the xCELLigence System

  1. Matthew Cooper3 and
  2. Lorenz M. Mayr4
  1. Yama A. Abassi1,
  2. Alexander Sieler2,
  3. Manfred Watzele2,
  4. Xiaobo Wang1 and
  5. Xiao Xu1

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch5

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Abassi, Y. A., Sieler, A., Watzele, M., Wang, X. and Xu, X. (2011) Dynamic and Label-Free Cell-Based Assays Using the xCELLigence System, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch5

Editor Information

  1. 3

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 4

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. 1

    ACEA Biosciences Inc., 6779 Mesa Ridge Rd, San Diego, CA 92121, USA

  2. 2

    Roche Diagnostics GmbH, BP-C1 Nonnenwald 2, 82377 Penzberg, Germany

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • dynamic and label-free cell-based assays - using the xCELLigence System;
  • xCELLigence System;
  • cell-based assays - of preclinical drug discovery process;
  • label-free cell-based technologies – implementation, in preclinical drug discovery settings;
  • actual kinetic response of cells - information regarding biological status of cells;
  • xCELLigence Real-Time Cell Analysis (RT-CA) system - series of three instruments differing primarily in throughput capacity;
  • xCELLigence system, and impedance readout - noninvasively quantifying cellular status in real time;
  • impedance readout utilized by xCELLigence system - affected by multiple parameters, cell number, adhesion and morphology

Summary

This chapter contains sections titled:

  • Introduction

  • The xCELLigence System

  • Principle of Detection

  • Applications

  • Functional Assays for G-Protein Coupled Receptors

  • Conclusion

  • References