7. Incorporating Transmitted Light Modalities into High-Content Analysis Assays

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Robert Graves

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch7

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Graves, R. (2011) Incorporating Transmitted Light Modalities into High-Content Analysis Assays, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch7

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. GE Healthcare Life Sciences, 800 Centennial Avenue, Piscataway, New Jersey 08855-1327, USA

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • high-content analysis assays and transmitted light modalities' incorporation;
  • high content cell-based assays - in research and pharmaceutical drug discovery;
  • high content image analysis software - data for each cell analyzed, measurements related to object intensity and morphology descriptors;
  • alternative approach - incorporating transmitted light imaging into high content analysis;
  • transmitted light (bright field) imaging;
  • IN cell investigator analysis software - with the IN Cell Analyzer 1000, and Developer toolbox;
  • high content analysis, application of high throughput automated microscopy - in concert with automated multiparameter image analysis;
  • transmitted light modalities - additional analysis capability, investigators avoiding use of harmful fluorescent stains

Summary

This chapter contains sections titled:

  • Introduction

  • Transmitted Light (Bright Field) Imaging

  • Image Analysis of Phase Contrast Images

  • Conclusion

  • References