8. Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels

  1. Matthew Cooper2 and
  2. Lorenz M. Mayr3
  1. Georg C. Terstappen

Published Online: 24 FEB 2011

DOI: 10.1002/9780470979129.ch8

Label-Free Technologies for Drug Discovery

Label-Free Technologies for Drug Discovery

How to Cite

Terstappen, G. C. (2011) Nonradioactive Rubidium Efflux Assay Technology for Screening of Ion Channels, in Label-Free Technologies for Drug Discovery (eds M. Cooper and L. M. Mayr), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470979129.ch8

Editor Information

  1. 2

    Institute for Molecular Bioscience, University of Queensland, Australia

  2. 3

    Biology Unit, Protease Platform, Novartis Pharma AG, Basel, Switzerland

Author Information

  1. Faculty of Pharmacy, University of Siena, Via Fiorentina 1, 53100 Siena, Italy

Publication History

  1. Published Online: 24 FEB 2011
  2. Published Print: 25 FEB 2011

ISBN Information

Print ISBN: 9780470746837

Online ISBN: 9780470979129

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Keywords:

  • nonradioactive rubidium efflux assay technology - for ion channel screening;
  • human genome sequencing - identifying 400 pore-forming ion channel genes;
  • ion channels as drug targets - drugs blocking Ca2+ channels, treatment of hypertension, angina pectoris and arrhythmia;
  • HTS assays, for target classes - G-protein coupled receptors (GPCRs) and enzymes;
  • ion channel drug discovery - less developed due to technical difficulties, developing assays;
  • nonradioactive rubidium efflux assay - based on atomic absorption spectrometry;
  • atomic absorption spectroscopy (AAS) - technology, detection of trace elements in environmental, biological and medical samples;
  • assay protocol for analysis of calcium-activated SK channels - screening of modulators of SK channels

Summary

This chapter contains sections titled:

  • Introduction

  • Ion Channels as Drug Targets

  • Ion Channel Assays and Screening

  • Nonradioactive Rubidium Efflux Assay Based on Atomic Absorption Spectrometry

  • A Typical Assay Protocol

  • Conclusions

  • References