Chapter 8. Intracellular Cytokine Detection by Flow Cytometry

  1. Uma Prabhakar Ph.D. and
  2. Marian Kelley Director of Compliance
  1. Julie G. Wilkinson M.S.1,
  2. Carlos A. Aparicio Ph.D.1 and
  3. Wade E. Bolton Ph.D. Vice President2

Published Online: 8 APR 2008

DOI: 10.1002/9780470987810.ch8

Validation of Cell-Based Assays in the GLP Setting: A Practical Guide

Validation of Cell-Based Assays in the GLP Setting: A Practical Guide

How to Cite

Wilkinson, J. G., Aparicio, C. A. and Bolton, W. E. (2008) Intracellular Cytokine Detection by Flow Cytometry, in Validation of Cell-Based Assays in the GLP Setting: A Practical Guide (eds U. Prabhakar and M. Kelley), John Wiley & Sons, Ltd, Chichester, UK. doi: 10.1002/9780470987810.ch8

Editor Information

  1. Department of Clinical Pharmacology & Experimental Medicine, Centocor Research and Development, Inc., 145 King of Prussia Road, Radnor, PA 19087, USA

Author Information

  1. 1

    Beckman Coulter, Inc, Custom BioPharma Solutions, 11800 SW 147th Ave., MC21-A01, Miami, FL 33196, USA

  2. 2

    Custom Bio/Pharma Solutions, Beckman Coulter, Inc., 4300 N. Harbor Blvd., (M/C E-34-E), Fullerton, CA 92835, USA

Publication History

  1. Published Online: 8 APR 2008
  2. Published Print: 21 MAR 2008

ISBN Information

Print ISBN: 9780470028766

Online ISBN: 9780470987810

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Keywords:

  • intracellular cytokine staining;
  • good laboratory practice;
  • peripheral blood mononuclear cells

Summary

This chapter contains sections titled:

  • Introduction

  • Assay standardization

  • Reagents/controls

  • Breaking down the ICS assay method

  • Assay reagents, controls, acquisition and analysis

  • Results

  • Conclusion

  • References