Chapter 2. Laboratory Techniques for the Identification of Abnormalities of Globin Chain Synthesis
- Barbara J. Bain MBBS FRACP FRCPath
Published Online: 12 NOV 2007
DOI: 10.1002/9780470988787.ch2
Copyright © 2006 by Blackwell Publishing Ltd
Book Title
Haemoglobinopathy Diagnosis, Second Edition
Additional Information
How to Cite
Bain, B. J. (ed) (2007) Laboratory Techniques for the Identification of Abnormalities of Globin Chain Synthesis, in Haemoglobinopathy Diagnosis, Second Edition, Blackwell Publishing Ltd, Oxford, UK. doi: 10.1002/9780470988787.ch2
Editor Information
Professor of Diagnostic Haematology, St Mary's Hospital Campus of Imperial College, Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary's Hospital, London
Publication History
- Published Online: 12 NOV 2007
- Published Print: 1 JAN 2006
ISBN Information
Print ISBN: 9781405135160
Online ISBN: 9780470988787
- Summary
- Chapter
- References
Keywords:
- anticoagulated blood;
- blood count;
- reticulocyte count;
- haemoglobin electrophoresis;
- cellulose acetate
Summary
This chapter contains sections titled:
Sample collection
Blood count, film and reticulocyte count
Preparing a red cell lysate
Haemoglobin electrophoresis
Isoelectric focusing
High performance liquid chromatography
Sickle solubility test and other methods for the detection of haemoglobin S
Quantification of haemoglobin A2
Immunoassay for variant haemoglobins
Quantification of haemoglobin F and determination of the distribution of haemoglobin F
Detection of haemoglobin H inclusions
Detection of an unstable haemoglobin
Detection of Heinz bodies
Osmotic fragility as a screening test for thalassaemia
2,6-Dichlorophenolindophenol test for haemoglobin E screening
Quantification of haemoglobin A1c
Other more specialized tests
Quality assurance
Check your knowledge
Further reading
Answers to questions
Appendix