Chapter 2. Laboratory Techniques for the Identification of Abnormalities of Globin Chain Synthesis

  1. Barbara J. Bain MBBS FRACP FRCPath

Published Online: 12 NOV 2007

DOI: 10.1002/9780470988787.ch2

Haemoglobinopathy Diagnosis, Second Edition

Haemoglobinopathy Diagnosis, Second Edition

How to Cite

Bain, B. J. (ed) (2006) Laboratory Techniques for the Identification of Abnormalities of Globin Chain Synthesis, in Haemoglobinopathy Diagnosis, Second Edition, Blackwell Publishing Ltd, Oxford, UK. doi: 10.1002/9780470988787.ch2

Editor Information

  1. Professor of Diagnostic Haematology, St Mary's Hospital Campus of Imperial College, Faculty of Medicine, London and Honorary Consultant Haematologist, St Mary's Hospital, London

Publication History

  1. Published Online: 12 NOV 2007
  2. Published Print: 1 JAN 2006

ISBN Information

Print ISBN: 9781405135160

Online ISBN: 9780470988787



  • anticoagulated blood;
  • blood count;
  • reticulocyte count;
  • haemoglobin electrophoresis;
  • cellulose acetate


This chapter contains sections titled:

  • Sample collection

  • Blood count, film and reticulocyte count

  • Preparing a red cell lysate

  • Haemoglobin electrophoresis

  • Isoelectric focusing

  • High performance liquid chromatography

  • Sickle solubility test and other methods for the detection of haemoglobin S

  • Quantification of haemoglobin A2

  • Immunoassay for variant haemoglobins

  • Quantification of haemoglobin F and determination of the distribution of haemoglobin F

  • Detection of haemoglobin H inclusions

  • Detection of an unstable haemoglobin

  • Detection of Heinz bodies

  • Osmotic fragility as a screening test for thalassaemia

  • 2,6-Dichlorophenolindophenol test for haemoglobin E screening

  • Quantification of haemoglobin A1c

  • Other more specialized tests

  • Quality assurance

  • Check your knowledge

  • Further reading

  • Answers to questions

  • Appendix