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UNIT 1B.1 Growing and Analyzing Static Biofilms
Published Online: 1 AUG 2011
DOI: 10.1002/9780471729259.mc01b01s22
Copyright © 2005 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Microbiology
Additional Information
How to Cite
Merritt, J. H., Kadouri, D. E. and O'Toole, G. A. 2011. Growing and Analyzing Static Biofilms. Current Protocols in Microbiology. 22:B:1B.1:1B.1.1–1B.1.18.
Publication History
- Published Online: 1 AUG 2011
- Published Print: AUG 2011
| Organism | Incubation temperature (°C) | Solvent for solubilization of stained biofilms | Reference |
|---|---|---|---|
| Agrobacterium tumefaciens | 28 | 100% dimethyl sulfoxide (DMSO) | Danhorn et al., 2004 |
| Escherichia coli | 25 | 80% ethanol/20% acetone | O'Toole et al., 1999 |
| Pseudomonas aeruginosa | 25-37 | 95% ethanol | O'Toole et al., 1999 |
| Pseudomonas aeruginosa | 25-37 | 30% acetic acid | Zegans et al., 2009 |
| Pseudomonas fluorescens | 25-30 | 95% ethanol | O'Toole et al., 1999 |
| Staphylococcus aureus | 37 | 33% glacial acetic acid | Stepanovic et al., 2001 |
| Streptococcus mutans | 37 | 95% ethanol or 100% DMSO | O'Toole et al., 1999 |
| Vibrio cholerae | 25-30 | 100% DMSO | O'Toole et al., 1999 |
