UNIT 1F.2 2-D Gel-Based Proteomic Approaches to Antibiotic Drug Discovery
Published Online: 1 AUG 2012
Copyright © 2005 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Microbiology
How to Cite
Raatschen, N. and Elisabeth Bandow, J. 2012. 2-D Gel-Based Proteomic Approaches to Antibiotic Drug Discovery. Current Protocols in Microbiology. 26:F:1F.2:1F.2.1–1F.2.16.
- Published Online: 1 AUG 2012
The global analysis of changes in the protein composition of bacterial cells in response to treatment with antibiotic agents grants insights into the physiological response of cells to inhibition of vital cellular functions. This unit gives an overview of how global proteomic studies can impact antibacterial drug discovery by identifying or validating compound mechanism of action and by increasing the confidence in the value of genes with unknown function as potential new targets. It describes the design and function of a reference compendium of proteomic responses to inhibition of vital cellular functions through antibacterial agents or genetic down-regulation of potential target genes. An overview of the workflow for two-dimensional gel electrophoresis-based experiments is also presented. Curr. Protoc. Microbiol. 26:1F.2.1-1F.2.16. © 2012 by John Wiley & Sons, Inc.
- two-dimensional gel electrophoresis;
- two-dimensional polyacrylamide gel;
- peptide mass fingerprinting;
- mechanism of action;
- conditional mutants;
- stress response