UNIT 6A.5 Detection, Isolation, and Identification of Vibrio cholerae from the Environment

  1. Anwar Huq1,
  2. Bradd J. Haley1,
  3. Elisa Taviani1,
  4. Arlene Chen1,
  5. Nur A. Hasan1,
  6. Rita R. Colwell1,2

Published Online: 1 AUG 2012

DOI: 10.1002/9780471729259.mc06a05s26

Current Protocols in Microbiology

Current Protocols in Microbiology

How to Cite

Huq, A., Haley, B. J., Taviani, E., Chen, A., Hasan, N. A. and Colwell, R. R. 2012. Detection, Isolation, and Identification of Vibrio cholerae from the Environment. Current Protocols in Microbiology. 26:A:6A.5:6A.5.1–6A.5.51.

Author Information

  1. 1

    Maryland Pathogen Research Institute, Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, College Park, Maryland

  2. 2

    University of Maryland Institute of Advanced Computer Studies, University of Maryland, College Park, Maryland, and Bloomberg School of Public Health, Johns Hopkins University, Baltimore, Maryland

Publication History

  1. Published Online: 1 AUG 2012


Recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens in the environment. These improvements and a downward trend in the cost of molecular detection methods have contributed to increased frequency of detection of pathogenic microorganisms where traditional culture-based detection methods have failed. Culture methods also have been greatly improved, and the confluence of the two suites of methods provides a powerful tool for detection, isolation, and characterization of pathogens. While molecular detection provides data on the presence and type of pathogens, culturing methods allow a researcher to preserve the organism of interest for “-omics” studies, such as genomic, metabolomic, secretomic, and transcriptomic analysis, which are rapidly becoming more affordable. This has yielded a clearer understanding of the ecology and epidemiology of microorganisms that cause disease. In this unit, we present commonly accepted methods for isolation, detection, and characterization of V. cholerae, providing more extensive knowledge of the ecology and epidemiology of this organism. This unit has been fully revised and updated from the earlier version with the latest knowledge and additional information not previously included. Curr. Protoc. Microbiol. 26:6A.5.1-6A.5.51. © 2012 by John Wiley & Sons, Inc.


  • Vibrio cholera;
  • isolation;
  • identification;
  • detection;
  • characterization