Miscellaneous

UNIT 8B.1 Laboratory Maintenance of Helicobacter Species

  1. Thomas G. Blanchard1,
  2. John G. Nedrud2

Published Online: 1 FEB 2012

DOI: 10.1002/9780471729259.mc08b01s24

Lab Protocol Title

Current Protocols in Microbiology

Current Protocols in Microbiology

Additional Information

How to Cite

Blanchard, T. G. and Nedrud, J. G. 2012. Laboratory Maintenance of Helicobacter Species. Current Protocols in Microbiology. 8B.1.1–8B.1.19.

Author Information

  1. 1

    Department of Pediatrics, University of Maryland, Baltimore, Maryland

  2. 2

    Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio

Publication History

  1. Published Online: 1 FEB 2012
  2. Published Print: FEB 2012
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Helicobacter species are Gram-negative bacteria that colonize the gastric or intestinal mucosa of many mammalian and avian hosts and induce histologic inflammation. The association of H. pylori with gastritis, peptic ulcer disease, and gastric cancers makes it a significant human pathogen. Animal models for these diseases are being used to explore the pathogenesis of H. pylori infection and in vaccine development. Both bacterial and host factors contribute to Helicobacter pathogenesis; therefore, the microbiology is very important. This unit describes how to culture the most commonly used gastric Helicobacter species, H. pylori, H. mustelae, and H. felis. Curr. Protoc. Microbiol. 24:8B.1.1-8B.1.19. © 2012 by John Wiley & Sons, Inc.

Keywords: Helicobacter; growth curve; morphological identification; blood agar preparation; pinch biopsy culture methods

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