UNIT 10E.3 Genetic Manipulation of Streptomyces Species
Published Online: 1 NOV 2010
Copyright © 2010 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Microbiology
How to Cite
Nybo, S. E., Shepherd, M. D., Bosserman, M. A. and Rohr, J. 2010. Genetic Manipulation of Streptomyces Species. Current Protocols in Microbiology. 19:10E.3.1–10E.3.26.
- Published Online: 1 NOV 2010
- Published Print: NOV 2010
This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR. Curr. Protoc. Microbiol. 19:10E.3.1-10E.3.26. © 2010 by John Wiley & Sons, Inc.
Keywords: Streptomyces; protoplast; conjugation; genomic DNA isolation; transformation