Miscellaneous

UNIT 10E.3 Genetic Manipulation of Streptomyces Species

  1. S. Eric Nybo,
  2. Micah D. Shepherd,
  3. Mary A. Bosserman,
  4. Jürgen Rohr

Published Online: 1 NOV 2010

DOI: 10.1002/9780471729259.mc10e03s19

Lab Protocol Title

Current Protocols in Microbiology

Current Protocols in Microbiology

Additional Information

How to Cite

Nybo, S. E., Shepherd, M. D., Bosserman, M. A. and Rohr, J. 2010. Genetic Manipulation of Streptomyces Species. Current Protocols in Microbiology. 19:10E.3.1–10E.3.26.

Author Information

  1. College of Pharmacy, University of Kentucky, Lexington, Kentucky

Publication History

  1. Published Online: 1 NOV 2010
  2. Published Print: NOV 2010
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This unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR. Curr. Protoc. Microbiol. 19:10E.3.1-10E.3.26. © 2010 by John Wiley & Sons, Inc.

Keywords: Streptomyces; protoplast; conjugation; genomic DNA isolation; transformation

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