Chapter 24. Challenges and Current Solutions in Proteomic Sample Preparations

  1. Yoshinori Mine2,
  2. Kazuo Miyashita3 and
  3. Fereidoon Shahidi4
  1. Feng Tao

Published Online: 12 JUN 2009

DOI: 10.1002/9780813807263.ch24

Nutrigenomics and Proteomics in Health and Disease: Food Factors and Gene Interactions

Nutrigenomics and Proteomics in Health and Disease: Food Factors and Gene Interactions

How to Cite

Tao, F. (2009) Challenges and Current Solutions in Proteomic Sample Preparations, in Nutrigenomics and Proteomics in Health and Disease: Food Factors and Gene Interactions (eds Y. Mine, K. Miyashita and F. Shahidi), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813807263.ch24

Editor Information

  1. 2

    Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, Canada

  2. 3

    Faculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, Japan

  3. 4

    Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada

Author Information

  1. Pressure BioSciences, Inc., 14 Norfolk Avenue, South Easton, MA 02375, USA

Publication History

  1. Published Online: 12 JUN 2009
  2. Published Print: 20 MAY 2009

Book Series:

  1. Functional Food Science and Technology Series

Book Series Editors:

  1. Fereidoon Shahidi

ISBN Information

Print ISBN: 9780813800332

Online ISBN: 9780813807263

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Keywords:

  • challenges and current solutions in proteomic sample preparations;
  • biomarkers - target proteins present in host cells or secreted in circulation;
  • identification of correlation between physiological or pathological state and apparent concentration of biomarker candidate;
  • protein–protein and protein–substrate interactions - for essential biological events and processes in cells;
  • interesting laser capture microdissection (LCM) technology;
  • blood, serum, plasma, and other body fluid;
  • proteome extraction and purification methods;
  • novel process using Pressure Cycling Technology Sample Preparation System (PCT SPS);
  • tissue proteome extractions using PCT SPS;
  • nanotechnology devices - potential to expand capabilities of proteomics

Summary

This chapter contains sections titled:

  • Overview

  • Selections of Samples for Proteomic Studies and Biomarker Discoveries

  • Sample Collection and Preanalytical Treatment

  • Proteome Extraction and Purification Methods

  • Enrichment and Detection of Proteins Useful in Proteomic Studies

  • Outlook of Solving Sample Preparation Issues in Proteomics

  • References