11. Mass Spectrometric Strategies for Identification and Characterization of Carbonylated Peptides and Proteins

  1. Giancarlo Aldini1,
  2. Kyung-Jin Yeum2,
  3. Etsuo Niki3 and
  4. Robert M. Russell4
  1. Marina Carini and
  2. Marica Orioli

Published Online: 4 AUG 2010

DOI: 10.1002/9780813814438.ch11

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

How to Cite

Carini, M. and Orioli, M. (2010) Mass Spectrometric Strategies for Identification and Characterization of Carbonylated Peptides and Proteins, in Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (eds G. Aldini, K.-J. Yeum, E. Niki and R. M. Russell), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813814438.ch11

Editor Information

  1. 1

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 2

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

  3. 3

    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577 Japan

  4. 4

    Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, Maryland 20892 USA

Author Information

  1. Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

Publication History

  1. Published Online: 4 AUG 2010
  2. Published Print: 10 SEP 2010

ISBN Information

Print ISBN: 9780813815350

Online ISBN: 9780813814438

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Keywords:

  • mass spectrometric strategies, for identification and characterization of carbonylated peptides and proteins;
  • carbonylation, irreversible, non-enzymatic modification of proteins - and change in activity or function;
  • protein carbonylation, by reactive carbonyl species (RCS) - polyunsaturated fatty acid peroxidation;
  • oxidative decomposition of polyunsaturated fatty acids (PUFAs) - chain reactions leading to formation of RCS;
  • advanced lipoxidation end-products (ALEs);
  • mass spectrometry strategies - for protein oxidative modification identification;
  • RCS characterization - modified proteins in biological matrices;
  • method for immunoaffinity capture of DNPH-derivatized HNE and MDA-adducted tryptic peptides;
  • precursor ion scanning - specific mass spectrometric detection methods, strategy in studying protein modifications;
  • carbonylated protein characterization in vitro section, conventional mass spectrometric approaches - and peptide and protein detection

Summary

This chapter contains sections titled:

  • Introduction

  • General and New Approaches for MS Characterization of Covalently Modified Proteins

  • Characterization of Carbonylated Proteins In Vitro: Reaction Mechanisms, Functional Studies, and Biomarkers Identification

  • Identification and Characterization of Carbonylated Proteins in Biological Matrices: From In Vitro to Ex Vivo Studies

  • Conclusions

  • References