12. Nitrotyrosine: Quantitative Analysis, Mapping in Proteins, and Biological Significance

  1. Giancarlo Aldini3,
  2. Kyung-Jin Yeum4,
  3. Etsuo Niki5 and
  4. Robert M. Russell6
  1. José M. Souza1,
  2. Silvina Bartesaghi2,
  3. Gonzalo Peluffo1 and
  4. Rafael Radi1

Published Online: 4 AUG 2010

DOI: 10.1002/9780813814438.ch12

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

How to Cite

Souza, J. M., Bartesaghi, S., Peluffo, G. and Radi, R. (2010) Nitrotyrosine: Quantitative Analysis, Mapping in Proteins, and Biological Significance, in Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (eds G. Aldini, K.-J. Yeum, E. Niki and R. M. Russell), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813814438.ch12

Editor Information

  1. 3

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 4

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

  3. 5

    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577 Japan

  4. 6

    Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, Maryland 20892 USA

Author Information

  1. 1

    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la República, Avda. General Flores 2125, 11800 Montevideo, Uruguay

  2. 2

    Departamento de Histología, Departamento de Bioquímica, and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la República, Avda. General Flores 2125, 11800 Montevideo, Uruguay

Publication History

  1. Published Online: 4 AUG 2010
  2. Published Print: 10 SEP 2010

ISBN Information

Print ISBN: 9780813815350

Online ISBN: 9780813814438

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Keywords:

  • nitrotyrosine, quantitative analysis, mapping in proteins and biological significance;
  • elucidating pathways and effects of 3-NT formation - in pathophysiological conditions;
  • 3-NT formation pathways in cells - under biologically relevant conditions;
  • tyrosine nitration, routes based on free radical pathways;
  • protein 3-NT, good immunogenic antigen - generation of polyclonal and monoclonal antibodies;
  • limits of quantitation and basal plasma levels of protein 3-nitrotyrosine;
  • mapping 3-nitrotyrosine in proteins;
  • protein tyrosine nitration increase, associated with pathogenesis in human and animal models of disease;
  • protein 3-NT, biomarker of nitroxidative stress - in disease processes;
  • identifying specific proteins, sites of nitration and biochemical mechanisms - linking protein tyrosine nitration to pathogenesis in causative ways

Summary

This chapter contains sections titled:

  • Introduction

  • Foundation of 3-Nitrotyrosine Formation

  • Tyrosine Analogs as Probes for Following Nitration and Oxidation Reactions

  • Methods for Quantification of 3-Nitrotyrosine in Biological Samples

  • Mapping 3-Nitrotyrosine in Proteins

  • Conclusions and Perspectives

  • Acknowledgements

  • References