14. Covalent Modifications of Albumin Cys34 as a Biomarker of Mild Oxidative Stress

  1. Giancarlo Aldini1,
  2. Kyung-Jin Yeum2,
  3. Etsuo Niki3 and
  4. Robert M. Russell4
  1. Giancarlo Aldini1,
  2. Kyung-Jin Yeum2 and
  3. Giulio Vistoli Ph.D.1

Published Online: 4 AUG 2010

DOI: 10.1002/9780813814438.ch14

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

How to Cite

Aldini, G., Yeum, K.-J. and Vistoli, G. (2010) Covalent Modifications of Albumin Cys34 as a Biomarker of Mild Oxidative Stress, in Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (eds G. Aldini, K.-J. Yeum, E. Niki and R. M. Russell), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813814438.ch14

Editor Information

  1. 1

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 2

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

  3. 3

    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577 Japan

  4. 4

    Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, Maryland 20892 USA

Author Information

  1. 1

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 2

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

Publication History

  1. Published Online: 4 AUG 2010
  2. Published Print: 10 SEP 2010

ISBN Information

Print ISBN: 9780813815350

Online ISBN: 9780813814438

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Keywords:

  • covalent modifications of albumin Cys34 - as mild oxidative stress biomarker;
  • Human serum albumin (HSA), abundant protein in plasma - 60% of total circulating proteins;
  • serum albumin concentration and mortality risk-inverse relationship;
  • albumin Cys34 and its oxidized forms - potential biomarkers of oxidative damage;
  • molecular mechanisms of mercaptoalbumin - as extracellular antioxidant, main oxidation products;
  • CYS34 acting - as an antioxidant;
  • Cys34, reactive antioxidant and detoxifying residues - of electrophilic species;
  • direct infusion MS, infusing solutions of isolated albumin dissolved in denaturing solvent;
  • LC-ESI-MS approach for identification - of unknown and predicted modifications of Cys34;
  • mercaptoalbumin, markers of antioxidant status

Summary

This chapter contains sections titled:

  • Introduction

  • Cys34 Reactivity: A Molecular Insight

  • Cys34 Acts as an Antioxidant

  • Cys34 As a Detoxifying Agent for Reactive Carbonyl Species

  • Cys34 Oxidized Forms

  • Analytical Strategies to Measure Mercaptoalbumin and Cys34 Oxidative Modifications

  • Mercaptoalbumin and the Reversible and Irreversible Oxidized Forms as Markers of Oxidative Damage

  • Mercaptoalbumin as a Marker of Antioxidant Status

  • Conclusions

  • Acknowledgments

  • References