16. DNA Oxidation, Antioxidant Effects, and DNA Repair Measured with the Comet Assay

  1. Giancarlo Aldini4,
  2. Kyung-Jin Yeum5,
  3. Etsuo Niki6 and
  4. Robert M. Russell7
  1. Mária Dušinská1,2 and
  2. Andrew R. Collins3

Published Online: 4 AUG 2010

DOI: 10.1002/9780813814438.ch16

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

How to Cite

Dušinská, M. and Collins, A. R. (2010) DNA Oxidation, Antioxidant Effects, and DNA Repair Measured with the Comet Assay, in Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (eds G. Aldini, K.-J. Yeum, E. Niki and R. M. Russell), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813814438.ch16

Editor Information

  1. 4

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 5

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

  3. 6

    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577 Japan

  4. 7

    Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, Maryland 20892 USA

Author Information

  1. 1

    CEE Health Effects Group, Norwegian Institute for Air Research, PB 100 2027 Kjeller, Norway

  2. 2

    Department of Experimental and Applied Genetics, Slovak Medical University, Limbova 12 833 03 Bratislava, Slovakia

  3. 3

    Department of Nutrition, University of Oslo, PB 1046 Blindern, 0316 Oslo, Norway

Publication History

  1. Published Online: 4 AUG 2010
  2. Published Print: 10 SEP 2010

ISBN Information

Print ISBN: 9780813815350

Online ISBN: 9780813814438

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Keywords:

  • DNA oxidation, antioxidant effects, and DNA repair measured with comet assay;
  • biomarker choice in human biomonitoring studies - nature of study and aims of investigation;
  • DNA oxidation, exposure to environmental mutagens – damage, being attack by endogenous free radicals;
  • comet assay, its application to study of environmental and occupational exposure to mutagens;
  • measuring DNA oxidation - as biomarker, chromatographic: GC-MS and HPLC with electrochemical detection;
  • comet assay limitations - and range of BER and NER activities in human lymphocytes;
  • comet assay, biomarker assay in studies of exposure of humans - to environmental mutagens/carcinogens;
  • link between fruit and vegetable consumption - reduced cancer incidence, attributed to high content of antioxidants;
  • oxidative DNA damage, measurable in human cells - by HPLC or FPG-based assays;
  • genetic polymorphism influence - on DNA damage and repair

Summary

This chapter contains sections titled:

  • Introduction

  • Principles of the Comet Assay and Applications to Biomarker Measurement

  • Measuring DNA Oxidation

  • Limitations of the Comet Assay

  • Assessing Antioxidant Status

  • Measuring DNA Repair

  • Some Considerations When Designing and Carrying Out a Biomonitoring Trial

  • Occupational, Environmental, and Experimental Exposure to Genotoxic Agents

  • Effects of Nutrition on DNA Stability

  • Influence of Genetic Polymorphisms on DNA Damage and Repair

  • Conclusion

  • Acknowledgments

  • References