17. Hydroxylated Nucleotides: Measurement and Utility as Biomarkers for DNA Damage, Oxidative Stress, and Antioxidant Efficacy

  1. Giancarlo Aldini2,
  2. Kyung-Jin Yeum3,
  3. Etsuo Niki4 and
  4. Robert M. Russell5
  1. Phyllis E. Bowen

Published Online: 4 AUG 2010

DOI: 10.1002/9780813814438.ch17

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications

How to Cite

Bowen, P. E. (2010) Hydroxylated Nucleotides: Measurement and Utility as Biomarkers for DNA Damage, Oxidative Stress, and Antioxidant Efficacy, in Biomarkers for Antioxidant Defense and Oxidative Damage: Principles and Practical Applications (eds G. Aldini, K.-J. Yeum, E. Niki and R. M. Russell), Wiley-Blackwell, Oxford, UK. doi: 10.1002/9780813814438.ch17

Editor Information

  1. 2

    Dipartimento di Scienze Farmaceutiche “Pietro Pratesi”, Universitàdegli Studi di Milano, Via L. Mangiagalli 25, 20133 Milan, Italy

  2. 3

    Jean Mayer USDA—Human Nutrition Research Center on Aging Tufts University, 711 Washington St., Boston, MA 02111 USA

  3. 4

    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 563-8577 Japan

  4. 5

    Office of Dietary Supplements, National Institutes of Health, 6100 Executive Blvd., Bethesda, Maryland 20892 USA

Author Information

  1. Department of Kinesiology and Nutrition, University of Illinois at Chicago, m/c 994, Chicago, IL 60612, USA

Publication History

  1. Published Online: 4 AUG 2010
  2. Published Print: 10 SEP 2010

ISBN Information

Print ISBN: 9780813815350

Online ISBN: 9780813814438

SEARCH

Keywords:

  • hydroxylated nucleotides - measurement and utility as biomarkers for DNA damage, oxidative stress and antioxidant efficacy;
  • 8OHdG, choice as biomarker of oxidative DNA damage - and as general marker of oxidative stress;
  • hydroxyl radical - common biological process;
  • hydroxylated guanine base (HGB) metabolism;
  • utility of understanding - measurement of guanine-based urinary excretion products, still promising;
  • damage to nuclear DNA (nDNA) - focus of research, because of mutation consequences;
  • European Standards Committee on Oxidative DNA damage (ESCODD);
  • DNA oxidation product identification - using GC-MS techniques;
  • seasonal effects on stability and absorption of food-based 8OHdG;
  • 8OHDG, as a predictor of disease

Summary

This chapter contains sections titled:

  • Introduction

  • Why Focus on the Measurement of 8-Hydroxydeoxyguanosine (8OHdG)?

  • Hydroxylated Guanine Base(HGB) Nomenclature

  • Hydroxylated Nucleotide Formation, Repair, and Metabolism

  • Where Have Hyroxylated Guanine Bases (HGB) Been Measured?

  • Methods of Measurement in Various Biological Compartments

  • The Practical use of Hydroxylated Bases as Biomarkers

  • Utility of 8OHDG as a Predictor of Disease

  • Utility of 8OHdG as a Measure of Environmental Insult

  • Utility as a Biomarker of Therapeutic Improvement

  • Conclusions and Suggestions

  • References