3. Multidimensional Liquid Chromatography: Theoretical Considerations

  1. Luigi Mondello
  1. Pavel Jandera

Published Online: 25 JUL 2011

DOI: 10.1002/9781118003466.ch3

Comprehensive Chromatography in Combination with Mass Spectrometry

Comprehensive Chromatography in Combination with Mass Spectrometry

How to Cite

Jandera, P. (2011) Multidimensional Liquid Chromatography: Theoretical Considerations, in Comprehensive Chromatography in Combination with Mass Spectrometry (ed L. Mondello), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118003466.ch3

Editor Information

  1. University of Messina, Messina, Italy

Author Information

  1. University of Pardubice, Pardubice, Czech Republic

Publication History

  1. Published Online: 25 JUL 2011
  2. Published Print: 26 AUG 2011

Book Series:

  1. Wiley-Interscience Series in Mass Spectrometry

Book Series Editors:

  1. Dominic M. Desiderio3 and
  2. Nico M. M. Nibbering4

Series Editor Information

  1. 3

    Departments of Neurology and Biochemistry, University of Tennessee Health Science Center, USA

  2. 4

    Vrije Universiteit Amesterdam, The Netherlands

ISBN Information

Print ISBN: 9780470434079

Online ISBN: 9781118003466



  • multidimensional liquid chromatography - chromatographic resolution, for reliable sample compound identification and determination;
  • orthogonality in two-dimensional LC–LC systems - maximum 2D peak capacity, with orthogonal separation systems;
  • separation selectivity, and phase system selection in two-dimensional LC–LC - 2D HPLC systems, different selectivities in first and second dimensions


This chapter contains sections titled:

  • Two-Dimensional LC Techniques

  • Peak Capacity in HPLC: One- and Multidimensional Separations

  • Orthogonality in Two-Dimensional LC–LC Systems

  • Sample Dimensionality and Structural Correlations

  • Separation Selectivity and Selection of Phase Systems in Two-Dimensional LC–LC

  • Programmed Elution in Two-Dimensional HPLC

  • Fraction Transfer Modulation in Comprehensive LC X LC: Additional Band Broadening

  • Future Perspectives