12. Assays for Evaluation of HIV-1 Integrase Enzymatic Activity, DNA Binding, and Cofactor Interaction

  1. Nouri Neamati
  1. Frauke Christ1,
  2. Katrien Busschots1,
  3. Jelle Hendrix2,
  4. Melissa McNeely1,
  5. Yves Engelborghs2 and
  6. Zeger Debyser3

Published Online: 28 SEP 2011

DOI: 10.1002/9781118015377.ch12

HIV-1 Integrase: Mechanism and Inhibitor Design

HIV-1 Integrase: Mechanism and Inhibitor Design

How to Cite

Christ, F., Busschots, K., Hendrix, J., McNeely, M., Engelborghs, Y. and Debyser, Z. (2011) Assays for Evaluation of HIV-1 Integrase Enzymatic Activity, DNA Binding, and Cofactor Interaction, in HIV-1 Integrase: Mechanism and Inhibitor Design (ed N. Neamati), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118015377.ch12

Editor Information

  1. Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy, Los Angeles, California, USA

Author Information

  1. 1

    Laboratory of Molecular Virology and Gene Therapy, Katholieke Universiteit Leuven, Leuven, Belgium

  2. 2

    Biochemistry, Molecular and Structural Biology, Katholieke Universiteit Leuven, Leuven, Belgium

  3. 3

    Laboratory of Molecular Virology and Gene Therapy, and Division of Molecular Medicine, Katholieke Universiteit Leuven, Flauders, Belgium

Publication History

  1. Published Online: 28 SEP 2011
  2. Published Print: 9 SEP 2011

Book Series:

  1. Wiley Series in Drug Discovery and Development

Book Series Editors:

  1. Binghe Wang

ISBN Information

Print ISBN: 9780470184745

Online ISBN: 9781118015377

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Keywords:

  • HIV-1 integrase enzymatic activity assay;
  • recombinant protein assays;
  • AlphaScreen technology

Summary

This chapter contains sections titled:

  • Introduction

  • Recombinant Protein Assays

  • Analysis of HIV-1 Integrase Cofactor Interactions

  • Cell-Based Assays to Study Virology and Cofactor Interaction of Integrase

  • Concluding Remarks

  • References