11. Biophysical Characterization of Protein Antigens within Vaccine Formulations

  1. Manmohan Singh and
  2. Indresh K. Srivastava
  1. Padma Malyala,
  2. Derek O'Hagan and
  3. Manmohan Singh

Published Online: 6 JUN 2011

DOI: 10.1002/9781118023648.ch11

Development of Vaccines: From Discovery to Clinical Testing

Development of Vaccines: From Discovery to Clinical Testing

How to Cite

Malyala, P., O'Hagan, D. and Singh, M. (2011) Biophysical Characterization of Protein Antigens within Vaccine Formulations, in Development of Vaccines: From Discovery to Clinical Testing (eds M. Singh and I. K. Srivastava), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118023648.ch11

Editor Information

  1. Novartis Vaccines, Cambridge, Massachusetts, USA

Author Information

  1. Novartis Vaccines, Cambridge, Massachusetts, USA

Publication History

  1. Published Online: 6 JUN 2011
  2. Published Print: 18 JUL 2011

ISBN Information

Print ISBN: 9780470256374

Online ISBN: 9781118023648

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Keywords:

  • protein antigen biophysical characterization - within vaccine formulations;
  • light-based methods, combined biophysical techniques of static (SLS) - and dynamic light scattering (DLS), protein states in formulations;
  • conjunction of biophysical tools, complex protein antigens in formulation - analytical ultracentrifugation, surface plasmon resonance (SPR)

Summary

This chapter contains sections titled:

  • Introduction

  • Chromatography-Based Methods

  • Light-Based Methods

  • Differential Scanning Calorimetry

  • X-Ray Powder Diffractometry

  • Summary

  • References