12. Structural Characteristics Predict the Stability of HIV

  1. Manmohan Singh and
  2. Indresh K. Srivastava
  1. Zohar Biron-Sorek1,
  2. Yide Sun2,
  3. Elaine Kan2,
  4. Jeanne Flandez3,
  5. Michael Franti3,
  6. Jeffrey Ulmer3,
  7. Susan Barnett3 and
  8. Indresh K. Srivastava3

Published Online: 6 JUN 2011

DOI: 10.1002/9781118023648.ch12

Development of Vaccines: From Discovery to Clinical Testing

Development of Vaccines: From Discovery to Clinical Testing

How to Cite

Biron-Sorek, Z., Sun, Y., Kan, E., Flandez, J., Franti, M., Ulmer, J., Barnett, S. and Srivastava, I. K. (2011) Structural Characteristics Predict the Stability of HIV, in Development of Vaccines: From Discovery to Clinical Testing (eds M. Singh and I. K. Srivastava), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118023648.ch12

Editor Information

  1. Novartis Vaccines, Cambridge, Massachusetts, USA

Author Information

  1. 1

    Weizmann Institute of Science, Rehovot, Israel

  2. 2

    Novartis Vaccines and Diagnostics, Emeryville, California, USA

  3. 3

    Novartis Vaccines, Cambridge, Massachusetts, USA

Publication History

  1. Published Online: 6 JUN 2011
  2. Published Print: 18 JUL 2011

ISBN Information

Print ISBN: 9780470256374

Online ISBN: 9781118023648

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Keywords:

  • formulation optimization and stability evaluation - predicting HIV stability, vaccine against AIDS, challenging;
  • primary obstacles, for effective vaccines - reactive neutralizing antibodies, against heterologous HIV-1 primary isolates;
  • analytical techniques, protein-based candidate vaccines - stability, circular dichroism (CD) and DSC

Summary

This chapter contains sections titled:

  • Introduction

  • Results

  • Discussion

  • Materials and Methods

  • Circular Dichroism

  • HPLC CD4 Binding Assay

  • Differential Scanning Calorimetric Analysis of Proteins

  • Hydrodynamic Radius Measurement

  • Surface Plasmon Resonance Assay

  • References