7. Expression and Purification of Recombinant Proteins for Vaccine Applications

  1. Manmohan Singh and
  2. Indresh K. Srivastava
  1. Carlo Zambonelli1,
  2. Deeann Martinez-Guzmann2 and
  3. Indresh K. Srivastava3

Published Online: 6 JUN 2011

DOI: 10.1002/9781118023648.ch7

Development of Vaccines: From Discovery to Clinical Testing

Development of Vaccines: From Discovery to Clinical Testing

How to Cite

Zambonelli, C., Martinez-Guzmann, D. and Srivastava, I. K. (2011) Expression and Purification of Recombinant Proteins for Vaccine Applications, in Development of Vaccines: From Discovery to Clinical Testing (eds M. Singh and I. K. Srivastava), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118023648.ch7

Editor Information

  1. Novartis Vaccines, Cambridge, Massachusetts, USA

Author Information

  1. 1

    Novartis Vaccines and Diagnostics, Cambridge, Massachusetts, USA

  2. 2

    Novartis Vaccines and Diagnostics, Emeryville, California, USA

  3. 3

    Novartis Vaccines, Cambridge, Massachusetts, USA

Publication History

  1. Published Online: 6 JUN 2011
  2. Published Print: 18 JUL 2011

ISBN Information

Print ISBN: 9780470256374

Online ISBN: 9781118023648

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Keywords:

  • recombinant proteins, expression and purification - for vaccine applications;
  • different expression vectors - prokaryotic, protein expression systems and expression vectors;
  • insect cell–baculovirus expression system - powerful platforms, and recombinant proteins, Spodoptera Frugiperda (SF) 9 or 21

Summary

This chapter contains sections titled:

  • Protein Expression

  • Prokaryotic Protein Expression Systems and Expression Vectors

  • Escherichia coli

  • Autoinduction

  • Eukaryotic Expression Systems Yeasts

  • Insect Cells

  • Mammalian Cells

  • Baby Hamster Kidney (BHK) Cells

  • Human Cells

  • Cell-Free Expression Systems

  • Protein Refolding

  • Protein Purification

  • References