7. Kinetics of Two-Substrate Enzymatic Reactions

  1. Ross L. Stein

Published Online: 27 MAY 2011

DOI: 10.1002/9781118084410.ch7

Kinetics of Enzyme Action: Essential Principles for Drug Hunters

Kinetics of Enzyme Action: Essential Principles for Drug Hunters

How to Cite

Stein, R. L. (2011) Kinetics of Two-Substrate Enzymatic Reactions, in Kinetics of Enzyme Action: Essential Principles for Drug Hunters, John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118084410.ch7

Publication History

  1. Published Online: 27 MAY 2011
  2. Published Print: 25 JUL 2011

ISBN Information

Print ISBN: 9780470414118

Online ISBN: 9781118084410

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Keywords:

  • kinetics of two-substrate enzymatic reactions - enzyme kinetic mechanism, drug discovery programs;
  • rate equation derivation - and sequential mechanisms;
  • ping-pong mechanism, differing - two sequential mechanisms, two irreversible chemical steps

Summary

This chapter contains sections titled:

  • Importance in Drug Discovery

  • Basic Mechanisms

  • Conceptual Understanding of Sequential Mechanisms

  • Derivation of Rate Equations for Sequential Mechanisms

  • Ping-Pong Mechanisms

  • Determining the Kinetic Mechanism for Two-Substrate Reactions

  • A Conceptual Understanding of the Shapes of Secondary Plots

  • Mistaken Identity: Rapid Equilibrium Random Versus Steady-State Ordered