7. Chemical Footprinting for Determining Protein Properties and Interactions

  1. Michael L. Gross2,
  2. Guodong Chen3 and
  3. Birendra N. Pramanik4
  1. Sandra A. Kerfoot1 and
  2. Michael L. Gross2

Published Online: 23 SEP 2011

DOI: 10.1002/9781118116555.ch7

Protein and Peptide Mass Spectrometry in Drug Discovery

Protein and Peptide Mass Spectrometry in Drug Discovery

How to Cite

Kerfoot, S. A. and Gross, M. L. (2011) Chemical Footprinting for Determining Protein Properties and Interactions, in Protein and Peptide Mass Spectrometry in Drug Discovery (eds M. L. Gross, G. Chen and B. N. Pramanik), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118116555.ch7

Editor Information

  1. 2

    Washington University, St. Louis, MO, USA

  2. 3

    Bristol-Myers Squibb, Princeton, NJ, USA

  3. 4

    Merck Research Laboratories, Kenilworth, NJ, USA

Author Information

  1. 1

    Seattle Childrens Research Institute, Seattle, WA, USA

  2. 2

    Washington University, St. Louis, MO, USA

Publication History

  1. Published Online: 23 SEP 2011
  2. Published Print: 21 OCT 2011

ISBN Information

Print ISBN: 9780470258170

Online ISBN: 9781118116555

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Keywords:

  • chemical footprinting;
  • hydrogen–deuterium exchange;
  • footprinting proteins

Summary

This chapter contains sections titled:

  • Introduction to Hydrogen–Deuterium Exchange

  • Experimental Procedures

  • Mass Spectrometry-Based HDX in Practice

  • Protein Footprinting via Free-Radical Oxidation

  • Chemical Crosslinking

  • Selective and Irreversible Chemical Modification

  • Conclusion

  • References