1. Targeted Proteomics Using Immunoaffinity Purification

  1. Mike S. Lee
  1. Karen R. Jonscher1,
  2. Lei Jin2,
  3. John C. Cambier3,
  4. Shaikh M. Rahman4 and
  5. Jacob E. Friedman5

Published Online: 21 MAY 2012

DOI: 10.1002/9781118180730.ch1

Mass Spectrometry Handbook

Mass Spectrometry Handbook

How to Cite

Jonscher, K. R., Jin, L., Cambier, J. C., Rahman, S. M. and Friedman, J. E. (2012) Targeted Proteomics Using Immunoaffinity Purification, in Mass Spectrometry Handbook (ed M. S. Lee), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118180730.ch1

Editor Information

  1. Milestone Development Services, USA

Author Information

  1. 1

    Department of Anesthesiology, University of Colorado School of Medicine, Aurora, CO, USA

  2. 2

    Integrated Department of Immunology, University of Colorado School of Medicine, National Jewish Medical and Research Center, Denver, CO, USA

  3. 3

    Integrated Department of Immunology, National Jewish Medical and Research Center, Denver, CO, USA

  4. 4

    Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO, USA

  5. 5

    Departments of Pediatrics, Biochemistry and Molecular Genetics, and Reproductive Sciences, University of Colorado School of Medicine, Aurora, CO, USA

Publication History

  1. Published Online: 21 MAY 2012
  2. Published Print: 18 APR 2012

Book Series:

  1. Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods

Book Series Editors:

  1. Mike S. Lee

Series Editor Information

  1. Milestone Development Services, USA

ISBN Information

Print ISBN: 9780470536735

Online ISBN: 9781118180730

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Keywords:

  • biotechnology/proteins, multimodular, driving cellular function;
  • targeted proteomics using immunoaffinity purification;
  • GeLC-MS/MS, in complex protein mixture analysis;
  • immunoaffinity purification;
  • hypothesis-driven research questions, and proteomics approaches

Summary

This chapter contains sections titled:

  • Introduction

  • Experimental Protocols

  • Applications of the Protocols

  • Conclusion

  • Acknowledgments

  • References