10. Hydrogen/Deuterium Exchange Mass Spectrometry (HDX MS) in the Studies of Architecture, Dynamics, and Interactions of Biopharmaceutical Products

  1. Mike S. Lee
  1. Igor A. Kaltashov,
  2. Cedric E. Bobst and
  3. Rinat R. Abzalimov

Published Online: 21 MAY 2012

DOI: 10.1002/9781118180730.ch10

Mass Spectrometry Handbook

Mass Spectrometry Handbook

How to Cite

Kaltashov, I. A., Bobst, C. E. and Abzalimov, R. R. (2012) Hydrogen/Deuterium Exchange Mass Spectrometry (HDX MS) in the Studies of Architecture, Dynamics, and Interactions of Biopharmaceutical Products, in Mass Spectrometry Handbook (ed M. S. Lee), John Wiley & Sons, Inc., Hoboken, NJ, USA. doi: 10.1002/9781118180730.ch10

Editor Information

  1. Milestone Development Services, USA

Author Information

  1. Department of Chemistry, University of Massachusetts - Amherst, Amherst, MA, USA

Publication History

  1. Published Online: 21 MAY 2012
  2. Published Print: 18 APR 2012

Book Series:

  1. Wiley Series on Pharmaceutical Science and Biotechnology: Practices, Applications and Methods

Book Series Editors:

  1. Mike S. Lee

Series Editor Information

  1. Milestone Development Services, USA

ISBN Information

Print ISBN: 9780470536735

Online ISBN: 9781118180730

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Keywords:

  • HDX MS in biopharmaceutical studies of their architecture, dynamics;
  • HDX and info, solvent protection of labile hydrogen atoms;
  • HDX, protein behavior as reflected by HDX MS;
  • HDX MS in IFN misfolding;
  • success of MS in drug conformation, adaptability to biopharmaceutical needs

Summary

This chapter contains sections titled:

  • Introduction

  • Technique Consideration

  • Conformational Properties of Protein Drugs Probed by HDX MS : Interferon-B1A (HDX MS As a Reporter of Protein Misfolding and/or Conformational Instability)

  • Protein Interaction with Physiological Partners and Therapeutic Targets Probed by HDX MS: Mechanistic Aspects of Transferrin-Receptor Interaction Revealed by HDX MS

  • Challenges and Future Directions

  • Acknowledgments

  • References